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新型纳米金蛋白质定位微试管芯片和计算与信号转导【2025|PDF|Epub|mobi|kindle电子书版本百度云盘下载】

LinWang，StefanWolfl，MinghuJiang编著著
出版社：北京：清华大学出版社
ISBN：9787302178309
出版时间：2008
标注页数：135页
文件大小：28MB
文件页数：144页
主题词：细胞－信号－转导－分子生物学－英文

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图书目录

Chapter 1 Introduction1

1.1 Antibody Array Technologies and Applications1

1.2 Protein Phosphorylation,Localization and Function3

1.3 Interaction Proteomics and Pathway Building4

1.3.1 BMP2 Signaling5

1.3.2 The Effect of STI571 on Cell Signaling11

Chapter 2 Technologies12

2.1 Materials12

2.1.1 Cell Lines12

2.1.2 Chemicals12

2.1.3 Antibodies12

2.1.4 Protein-Molecular Weight Marker13

2.1.5 Apparatus13

2.2 Methods14

2.2.1 Cell Culture14

2.2.2 Cell Numbers and Experimental Format14

2.2.3 Cytoplasmic and Nuclear Protein Extracts14

2.2.4 Determination of Protein Concentration15

2.2.5 Ponceau S Staining15

2.2.6 Western Analysis16

2.2.7 Immunoprecipitation16

2.2.8 Cell Cycle Analysis16

2.3 Silver Staining in ArrayTube17

2.3.1 Biotinylation of Cellular Proteins17

2.3.2 Incubation of Protein Extracts17

2.3.3 Silver Staining17

2.3.4 Statistical Analysis18

Chapter 3 New Cytoplasmic and Nuclear BMP2-Signaling Pattern in U937 Cells23

3.1 Modulation of Phosphorylation and Localization in BMP2-Treated U937 Cells23

3.1.1 Total p-Tyrosine and p-p38,p38 Increased:Increasing Cytoplasm and Nucleus23

3.1.2 Total p-ERK and p-JNK Increased:Increasing Cytoplasm and Unchanged Nucleus25

3.1.3 Total p-Akt Increased:Increasing Cytoplasm and Decreasing Nucleus26

3.1.4 Total p-Smad1,p-Smad2/3,c-Myc and p-STAT3 Unchanged:Decreasing Cytoplasm and Increasing Nucleus29

3.1.5 Total p-p70S6 Unchanged:Increasing Cytoplasm and Decreasing Nucleus32

3.2 BMP2 Signaling in U937 Cells32

3.2.1 BMP2 Activates Akt,ERK and JNK Pathway in U937 Cells33

3.2.2 BMP2 Activates Smad1 and Smad2/3 Networks,p38 Network and c-Myc,Tyrosine Network in U937 Cells35

3.2.3 BMP2 Inhibits p70S6 Signaling in U937 cells36

3.3 BMP2 Signaling Model in U937 Cells38

Chapter 4 New Cytoplasmic and Nuclear BMP2-Signaling in MCF7 Cells40

4.1 Modulation of Phosphorylation and Localization in BMP2-Treated MCF7 Cells40

4.1.1 Total p-JNK,p-Smad1,p-Smad2/3,p-p38 and p38 Unchanged:Increasing Cytoplasm and Decreasing Nucleus41

4.1.2 p-Tyrosine,p-STAT3,p-ERK and p-p70S6 Increased:Increasing Cytoplasm and Unchanged Nucleus45

4.1.3 Total c-Myc Unchanged:Slightly Increasing Nucleus and Slightly Decreasing Cytoplasm46

4.1.4 Total p-Akt Unchanged:Unchanged Nucleus and Cytoplasm46

4.2 BMP2 Signaling in MCF7 Cells48

4.2.1 BMP2 Activates p70S6 and ERK Signaling,Tyrosine and STAT3 Network in MCF7 Cells48

4.2.2 BMP2 Inhibits p38,JNK and Smad Signaling in MCF7 Cells49

4.2.3 BMP2 Activates c-Myc Signaling in MCF7 Cells50

4.3 BMP2 Signaling Model in MCF7 Cells50

Chapter 5 New Cytoplasmic and Nuclear STI571 Signaling in K562 Cells52

5.1 Modulation of Phosphorylation and Localization in STI571-Treated K562 Cells52

5.1.1 Total p-Akt and p-p70S6 Decreased:Decreasing Cytoplasm and Nucleus52

5.1.2 Total p-p38,p38,c-Myc,p-Tyrosine,p-Smad1 and p-Smad2/3 Increased:Increasing Cytoplasm and Nucleus55

5.1.3 Total p-STAT3 and p-JNK Increased:Increasing Cytoplasm and Unchanged Nucleus56

5.1.4 Total p-ERK Increased:Decreasing Cytoplasm and Increasing Nucleus58

5.2 STI571 Signaling in K562 Cells58

5.2.1 STI571 Inhibits Akt/p70S6 PI3K Signaling in K562 Cells58

5.2.2 STI571 Activates p38 MAPK Pathway,c-Myc,Tyrosine and Smad1/Smad2/Smad3 Networks in K562 Cells59

5.2.3 STI571 Activates ERK Pathway in K562 Cells59

5.2.4 STI571 Activates STAT3 Network and JNK/MAPK Pathway in K562 Cells60

5.3 STI571 Signaling Model in K562 Cells61

Chapter 6 Positive and Negative Co-localized Relationships in Three Treated Cell Lines63

6.1 Positive and Negative Co-localized Distributions63

6.1.1 p-p38 and p3863

6.1.2 Smad1 and Smad2/363

6.1.3 Akt,ERK and p70S663

6.1.4 p70S6 with p38 and Smad1,2,375

6.1.5 ERK or p38 with Smad180

6.1.6 ERK or p38 with c-Myc81

6.2 Possible Relationships83

Chapter 7 Cytoplasmic Signaling in Three Treated Cell Lines87

7.1 Cytoplasmic Distribution87

7.1.1 p-ERK,p-JNK and p-Akt in BMP2-Induced Apoptosis in U937 Cells87

7.1.2 p-STAT3 and p-JNK in STI571-Treated K562 Cells87

7.1.3 p-Tyrosine,p-STAT3,p-ERK and p-p70S6 in BMP2-Treated MCF7 Cells91

7.2 Co-cytoplasmic Signaling92

Chapter 8 Proteomic Silencing in Treated Human Cancer Cell Lines95

8.1 Proteomic Silence Distributions95

8.1.1 p-p70S6 Nuclear Decrease in BMP2-Induced Apoptosis in U937 Cells95

8.1.2 p-ERK/p-JNK/p-p38,p-Smad1 and p-Smad2/3 Co-nuclear Decrease in BMP2-Treated MCF7 Cells98

8.1.3 p-Akt and p-p70S6 Co-cytoplasmic and Co-nuclear Decrease in STI571-Treated K562 Cells98

8.2 Possible Relationships98

Chapter 9 Signaling Pathway Setting-up Based on Bioinformatics100

9.1 Methods100

9.1.1 Mean and Variance100

9.1.2 Hierarchical Clustering101

9.1.3 Competitive Learning Network101

9.1.4 Self-Organizing Map(SOM)104

9.2 Results106

9.2.1 Mean and Variance Analysis of Cytoplasmic Signaling in Control and STI571-Treated K562 cells106

9.2.2 Competitive Learning Network Analysis of Cytoplasmic Signaling in Control and STI571-Treated K562 cells107

9.2.3 Hierarchical Clustering Analysis of Cytoplasmic Signaling in Control and STI571-Treated K562 cells109

9.2.4 SOM Analysis of Cytoplasmic Signaling in Control and STI571-Treated K562 cells110

9.3 Discussion117

9.4 Conclusions118

References119